How Cord Blood is Processed and Stored


Processing of your baby’s cord blood

Storing of your baby’s cord blood



Processing  your baby’s cord blood

Stem cell isolation is a critical step in cord blood banking. It affects the number of stem cells that can be harvested or recovered from the cord blood. Cell recovery rates are critical because a higher number of stem cells could enhance the success of the transplant or treatment. That's why Cordlife has invested in the Sepax® automated stem cell processing technology, a safe, sterile and fully automated cord blood processing technology – so you get more stem cells for maximum transplant success˄.

The first private cord blood bank in Singapore that offers the Sepax® automated processing technology, this Swiss-made and U.S. FDA-cleared device can recover as high as 99%1 of Total Nucleated Cells (TNC) from cord blood; up to 57%2 cell count higher than other processing methods. It is also a functionally closed processing system which ensures the sterility of the cord blood by eliminating exposure to air contaminants.


References
1. Cordlife umbilical cord blood processing data as at 31 December 2010
2. Valeri et al., Transfusion 1996; 36: 303-8.

back to top


Storing your baby’s cord blood

Cordlife uses the U.S. FDA approved cryogenic storage pouch. This multi-compartmentalised cryobag has 20% and 80% compartments. This cryobag also has 2 integrally attached segments which are in compliance with AABB and FACT-Netcord standards. This dual compartment addresses the stem cell expansion programmes in future. That means that when this stem cell expansion technology is commercially viable, you can withdraw 80% of the stem cells for immediate use /treatment while continuing to store the 20% balance for later expansion.

The integral segments also provide the safety and assurance that additional product testing is performed on the associated unit thereby eliminating testing and cord blood unit mix ups. This is to allow for additional testing of the sample should it be required in the future, or for viability testing prior to a transplant. This storage pouch is made of a special material designed specifically to withstand cryogenic temperatures.


Image 1 - Cordlife's FDA approved dual compartmental storage pouch
with integrated segments (before detachment)

Your baby’s cord blood is stored in vacuum insulated cryogenic storage tanks, and are designed to remain at optimal cryogenic temperature of below -150° for cell storage over long periods.

Cordlife uses MVE Anti-contamination Vapour-phase Liquid Nitrogen Storage System for long-term stem cell cryopreservation.

Vapour-phase storage is preferred over liquid-phase storage1 as it eliminates the chance of cross contamination between cord blood units as compared with those stored in liquid nitrogen.

The MVE Anti-contamination Vapour-phase Liquid Nitrogen Storage System operates perfectly, even without electrical supply, and is 100% reliable and failsafe with no chance of robotic malfunction. Unlike other storage systems available in the market today, our tanks have never been recalled by the U.S. FDA or the manufacturer.

Over 99% of private cord blood banks also use only vapour-phase freezers. Presently, the majority of stem cell transplantations used cord blood units stored in vapour freezers.


Image 2 - MVE Anti-contamination Vapour-phase
Liquid Nitrogen Storage at Cordlife storage facility

Reference:
1. Best practices for Repositories I: Collection, Storage, and Retrieval of Human Biological Materials for Research

back to top